Lineweaver-Burk Plot of Noncompetitive Inhibition
Shown on the next page is a plot of the reciprocal of enzymatic reaction velocity (1/v)
versus the reciprocal of substrate concentration (1/[S]). This Lineweaver-Burk
plot will portray the graphical consequences of competitive inhibition.
This plot is interactive, and the initial values of Km and Vmax are shown in
the box below the plot. The units for these parameters are also indicated.
When you click the "Plot [I]=" button, a second curve will be drawn. This
curve
is generated using the first of the inhibitor concentrations indicated in the
box below the plot. Clicking on the same button again
will generate the curve corresponding to the second inhibitor concentration.
Click again and the curve corresponding to the third inhibitor concentration will
be displayed.
To see how the plot changes when Km, Ki and [I] are varied, enter your choice of
values in the input boxes and click on "Plot Your Values." You may
enter as many
combinations of the three parameters as you like, but note that your values must
lie within a factor of ten of the initial values.
To begin the exercise again from the beginning, click on "Reset".
