Hanes-Woolf Plot of Competitive Inhibition
Shown on the next page is a plot of substrate concentration divided by enzymatic
reaction velocity ([S]/v) versus substrate concentration ([S]). This Hanes-Woolf
plot will portray the graphical consequences of competitive inhibition.
This plot is interactive, and the initial values of Km and Vmax are shown
in the box below the plot. The units for these parameters are also indicated.
When you click the "Plot [I]=" button, a second curve will be drawn. This
curve
is generated using the first of the inhibitor concentrations indicated in the
box below the plot. Clicking on the same button again
will generate the curve corresponding to the second inhibitor concentration.
Click again and the curve corresponding to the third inhibitor concentration
will be displayed.
To see how the plot changes when Km, Ki and [I] are varied, enter your choice
of values in the input boxes and click on "Plot Your Values." You
may
enter as many
combinations of the three parameters as you like, but note that your values must
lie within a factor of ten of the initial values.
To begin the exercise again from the beginning, click on "Reset".
