Hanes-Woolf Plot
Linear forms of the Michaelis-Menten equation provide interesting
insights
into enzyme kinetic data. In this exercise, we will examine a typical dataset and
the Hanes-Woolf plot of the data. We will then ask what happens when the Km and Vmax
parameters change.
Shown on the next page is a plot of substrate divided by enzymatic reaction velocity
([S]/v) versus substrate concentration ([S]), a Hanes-Woolf Plot.
This plot is interactive, and the initial values of Km and Vmax are shown
in the box below the plot. The units for these parameters are also indicated.
To see how the plot changes when Km and Vmax are changed by factors of
two, click the appropriate button. Then, to make your own choices for the
kinetic parameters, enter your values in both of the input boxes and click
on "Plot Your Values". Note that your values must lie within a factor of ten of the
initial values.
To begin the exercise again from the beginning, click on "Reset".
